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Measles virus (ATCC® VR-24)

Classification: Paramyxoviridae, Morbillivirus  /  Product Format: frozen 1 mL per vial

Classification Paramyxoviridae, Morbillivirus
Agent Measles virus
Strain Edmonston
A CE adapted Edmonston strain has been used for the production of vaccines for human use.
Respiratory research
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments Measles, Rinderpest and Canine distemper viruses contain a common antigen.
Effect on Host
CPE, syncytial rounding and sloughing
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 4-14 days
Effect on Host
CPE, syncytial rounding and sloughing
Name of Depositor JF Enders, Boston Children’s Hospital, Boston, Massachusetts
Special Collection NCRR Contract
Source Blood from patient in acute phase of typical measles, Massachusetts, 1954

Enders JF, Peebles TC. Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proc. Soc. Exp. Biol. Med. 86: 277-286, 1954. PubMed: 13177653