Penicillin-Streptomycin-Amphotericin B Solution

1. Take the vial from storage and thaw. Mix well or vortex briefly to make sure the solution is homogenous.
2. Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the appropriate volume of the antibiotic/antimycotic solution to complete media. 
3. Dilute 1:1000 when using to supplement complete growth media; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 500 mL of complete cell-specific media.
4. Dilute 1:100 when using during cell isolation procedures; i.e., 0.5 mL of Penicillin-Streptomycin-Amphotericin B Solution per 50 mL of complete cell-specific growth media.
5. Tightly cap the bottle (or other flask) of supplemented growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the flask.
6. Fully supplemented media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, fully supplemented media is stable for 30 days.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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