35210 ™
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Broth growth is non-turbid and is best observed via a wet mount. Organism does not grow well on agar.
Growth usually occurs after 4-5 days. Acid formation during growth will change the medium to a light or yellowish-orange color. Turbidity is not evident. Cells can be monitored under phase microscopy as long spiral rods, and their motility by the twitching movement.
Borrelia burgdorferi is a fragile, sensitive organism that must have the appropriate medium for growth. Rabbit serum is essential for the growth of this organism. Fresh medium enhances growth. Medium older than one month should not be used.
Purified genomic DNA of this strain is available as ATCC 35210D-5 and ATCC 35210DQ.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Johnson RC, et al. Borrelia burgdorferi sp. nov.: Etiologic agent of Lyme disease. Int. J. Syst. Bacteriol. 34: 496-497, 1984.
Baranton G, et al. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis. Int. J. Syst. Bacteriol. 42: 378-383, 1992. PubMed: 1380285
Barbour AG. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med. 57: 521-525, 1984. PubMed: 6393604
Takayama K, et al. Absence of lipopolysaccharide in the Lyme disease spirochete, Borrelia burgdorferi. Infect. Immun. 55: 2311-2313, 1987. PubMed: 3623705
Johnson SE, et al. Borrelia burgdorferi: survival in experimentally infected human blood processed for transfusion. J. Infect. Dis. 162: 557-559, 1990. PubMed: 2373880
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