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Geotrichum candidum Link : Persoon, anamorph

34614

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An ampoule containing viable cells (may include spores and mycelia) suspended in cryoprotectant.
Product category
Fungi
Product type
Yeast
Preceptrol culture
Type strain
No
Genome sequenced strain
Yes
Isolation source
Clotted carrot
Geographical isolation
Japan
Applications
Quality control
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Degrades sauerkraut brine
Produces acetoin dehydrogenase diacetyl reductase
Produces lactate dehydrogenase
Produces lipase
Produces polygalacturonase endopolygalacturonase, pectinase
Quality control strain
Produces lipases I and II
Produces type A lipase
Produces extracellular lipase from sauerkraut brine
Produces acetoin dehydrogenase from sauerkraut brine
Preceptrol
Yes

Characteristics

Morphology
On YM medium at 25°C after 3 days, colonies white to dingy white, finely hairy (able to see the branching hyphae in the texture), dense, raised. Hyphae hyaline, guttulate. Arthroconidia rectangular to cubic, hyaline, smooth, 6-7.5 X 3-3.75 µm. Yeast cells subglobose to oval, hyaline, smooth, larger cells 9-12 X 4.5-8.25 µm, smaller cells 5.25-6 X 4.5-5.25 µm.
Comments
Crystal structure of lipase
cDNA clone of lipase genes
The strain was recently re-identified as Galactomyces candidus (anamorphic synonym: Geotrichum candidum) based on the multigene sequence analysis.

Handling information

Medium
Temperature
24-26°C
Atmosphere
Aerobic
Handling procedure

For freeze-dry (lyophilized) ampoules:

  1. Open an ampoule according to enclosed instructions.
  2. From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
  3. Aseptically transfer the suspension back into the test tube of sterile distilled water.
  4. Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
  5. Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
  6. Incubate the inoculum at the propagation conditions recommended.
  7. Inspect for growth of the inoculum/strain regularly. Viability is typically noticeable after 1 to 2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.

 

Handling notes

Quality control strain.

Additional, updated information on this product may be available on the ATCC® web site at www.atcc.org.

Quality control specifications

Sequenced data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28/26S ribosomal RNA gene, partial sequence

AAGGATCATTAAGAATTAWWAATATTTGTGAAATTTACACAGCMAACAATAATTTTATAGTCAAAACAAAAATAATCAAAACTTTTAACAATGGATCTCTTGGTTCTCGTATCGATGAAGAACGCAGCGAAACGCGATATTTCTTGTGAATTGCAGAAGTGAATCATCAGTTTTTGAACGCACATTGCACTTTGGGGTATCCCCCAAAGTATACTTGTTTGAGCGTTGTTTCTCTCTTGGAATTGCATTGCTTTTCTAAAATTTCGAATCAAATTCGTTTGAAAAACAACACTATTCAACCTCARATCAAGTAGGATTACCCGCTGAACTTAAG


D1D2 region of the 28/26S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCGGCCMCCAGGTCGAGTTGTAATTTGTAGATTGTATCTTGAGAGCGGATTAAAGTCTGTTGGAACACAGCGCCTTAGAGGGTGACAGCCCCGTAAAATCTATTCTCATTGTAAGATACTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGAGGTAAATTCCTTCTAAAGCTAAATATTGACGAGAGACCGATAGCGAACAAGTACTGTGAAGGAAAGATGAAAAGCACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTAAAAGGGAAGGGTATTGAATCAGACTTGGTGCTGTTGTTCAACTGTGTTTCGGCATAGTGTACTCAGCAGTACTAGGCCAAGGTGGGGTGTTTGGGAGTGAAAAAGAAGTWGGAACGTAACTCTTCGGAGTGTTATAGCCTACTTTCATAGCTCCTCAGGCGCCTCAGGACTGCGCTTCGGCAAGGACCTTGGCATAATGATTCTATACCG

Verification method
Whole-genome Sequencing

History

Deposited as
Geotrichum candidum Link : Persoon
Synonyms
Oospora lactis (Fresenius) Saccardo, anamorph; Oidium matalense Castellani, anamorph; Oidium asteroides Castellani, anamorph; Geotrichum javanese Verona, anamorph; Geotrichum matalense Castellani, anamorph;
Depositors
M Iwai
Type of isolate
Food & Beverage; Plant
Cross references
GenBank KU933438 ITS including 5.8S rRNA gene
GenBank KU933412 D1/D2 region of 28S rRNA gene
GenBank KU933403 actin gene
GenBank E02497 cDNA encoding Geotrichum candidum lipase.
GenBank U02622 Geotrichum candidum ATCC 34614 lipase gene, partial cds.
GenBank U26858 Galactomyces geotrichum isolate g56 28S rRNA gene, partial
GenBank U02541 Geotrichum candidum ATCC 34614 lipase II gene, partial cds.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

. . Food Chem. 45: 15-17, 1992.

Hang YD, Woodams EE. Production and characterization of polygalacturonase from Geotrichum candidum. World J. Microbiol. Biotechnol. 8: 480-482, 1992.

Jacobsen T, Poulsen OM. Comparison of lipases from different strains of the fungus Geotrichum candidum. Biochim. Biophys. Acta 1257: 96-102, 1995. PubMed: 7619863

Shimada Y, et al. cDNA molecular cloning of Geotrichum candidum lipase. J. Biochem. 106: 383-388, 1989. PubMed: 2481674

Nagao T, et al. Cloning and sequencing of two chromosomal lipase genes from Geotrichum candidum. J. Biochem. 113: 776-780, 1993. PubMed: 8370674

View All Curated Citations for this Product

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