Clostridium lacusfryxellense Spring et al.
BAA-580 ™
- Product category
-
Bacteria
- Product type
-
Extremophile
- Strain designation
- DSM 14205 [C/C-an/B1]
- Type strain
- Yes
- Isolation source
- Microbial mat, McMurdo Dry Valley
- Geographical isolation
- Antarctica; Victoria Land
- Product format
- Frozen
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
-
Biotechnology
- Preceptrol
- No
Characteristics
- Extremophile type
-
Psychrophile
Handling information
- Medium
- Temperature
-
10°C
- Atmosphere
-
Anaerobic
- Handling procedure
-
1. This culture must be propagated under strictly anaerobic, conditions (see below).
2. Open the frozen vial and immediately place the vial under a stream of sterile gas, to maintain anaerobicity.
3. Under anaerobic conditions, withdraw the cell suspension from the vial and transfer it to a single test tube of ATCC #1490 broth.
4. A second broth tube, a slant and a pre-reduced blood plate may be inoculated with 0.1 ml each of the cell suspension. Also inoculate a non-selective plate and tube of broth to test for the presence of aerobic contaminates.
5. Incubate tubes and plate under anaerobic conditions at 10°C. Incubate aerobic plate(s) and broth tube(s) at 30oC.
6. Within 7 to 10 days, growth is evident by turbidity in broth and in the inoculum at the base of the slant. No growth should occur on blood agar plates incubated aerobically.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
- Handling notes
-
Always use freshly prepared pre-reduced media or pre-reduced media that has been previously prepared but stored under anaerobic conditions. Resazurin in the media is a color indicator for anaerobic conditions. Observance of pink color in medium before use or during incubation shows anaerobic conditions have not been met and oxidation has occurred. Medium should be discarded.
History
- Deposited as
- Clostridium lacusfryxellense Spring et al.
- Depositors
- DSMZ, H Hippe, A Hagelstein
- Chain of custody
- DSMZ
- Type of isolate
- Environmental
- Year of origin
- 1999
- Special collection
-
NSF - Bacteriology
- Cross references
-
GenBank AJ506118 Clostridium lacusfryxellense 16S rRNA gene, type strain DSM 14205.
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Brambilla E, et al. 16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell, McMurdo Dry Valleys, Antarctica. Extremophiles 5: 23-33, 2001. PubMed: 11302499
microbial mat sample from shallow, moated area, around Lake Fryxell, an antarctic freshwater lake, McMurdo Dry Valleys, Antarctica
Spring S, et al. Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov.. Int. J. Syst. Evol. Microbiol. 53: 1019-1029, 2003. PubMed: 12892121
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