Roseospira marina Guyoneaud et al.
BAA-447 ™
- Product category
-
Bacteria
- Product type
-
Extremophile
- Strain designation
- CE2105 [DSM 15113]
- Type strain
- Yes
- Isolation source
- Lagoon; isolated September 1992
- Geographical isolation
- France
- Product format
- Freeze-dried
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
-
Biotechnology
- Preceptrol
- No
Characteristics
- Extremophile type
-
Phototroph
Handling information
- Medium
- Temperature
-
26°C
- Atmosphere
-
Anaerobic
- Incubation
- Under light
- Handling procedure
-
1. Prepare test tube(s) of medium for inoculation by:
a) Exchanging the gas in the head space for 100% N2.
b) Adding Na-acetate to a final concentration of 2mM.
2. Open vial according to enclosed instructions.
3. With an anaerobic (see below) 1.0 ml syringe with draw 0.5 ml of pre-reduced ATCC Medium #2331 and rehydrate the entire pellet. Draw the rehydrated culture into the syringe and inject it into the pre-reduced tube of #2331 medium. A portion (0.5 ml) of the culture should be transferred to a second tube of pre-reduced medium #2331. All steps should be carried out using good anaerobic techniques. Mix well.
4. Use several drops of the suspension to inoculate a non-selective plate and incubate at 26oC in the dark.
5. Incubate the tubes and plate at 26oC for 7 to 10 days hours.
ANAEROBIC CONDITIONS:
A. Balch tubes (available from Bellco Glass, Vineland, NJ; are specially designed for anaerobic work and use an aluminum crimp cap to hold a rubber stopper in place. Needles can easily be inserted through the stopper, and the tubes can be pressurized. Alternatively, serum vials may be used, or screw cap tubes with butyl rubber stoppers, in the latter case the stopper may be removed and the tube placed under a cannula system that dispenses sterile, oxygen free gas for addition of reducing agents or inoculation.
C. Syringes can be made anaerobic by one of two methods.
- Handling notes
-
There should be no growth on the non-selective medium incubated aerobically in the dark.
When grown anaerobically under light the culture will exhibit a red pigmentation.
The cells are spiral to vibroid-shaped, motile and stain Gram negative.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
History
- Deposited as
- Roseospira marina
- Depositors
- R Guyoneaud
- Type of isolate
- Environmental
- Year of origin
- 1992
- Special collection
-
NSF - Bacteriology
- Cross references
-
GenBank AJ298879 Roseospira marina partial 16S rRNA gene, strain CE2105.
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
type strain
Guyoneaud R, et al. Characterization of three spiral-shaped purple nonsulfur bacteria isolated from coastal lagoon sediments, saline sulfur springs, and microbial mats: emended description of the genus Roseospira and description of Roseospira marina sp. nov., Roseospira navarrensis sp. nov., and Roseospira thiosulfatophila sp. nov.. Arch. Microbiol. 178: 315-324, 2002. PubMed: 12375098
. Validation of publication of new names and new combinations previously effectively published outside the IJSEM. Int. J. Syst. Evol. Microbiol. 53: 1701-1702, 2003. PubMed: 14657096
man-made brackish shallow lagoon, rich in organic matter and sulfide, that is periodically flooded with seawater, Certes Lagoon, Arcachon Bay
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