Moorella perchloratireducens

1. Sterilize the tops of the all test tubes by spraying them with 70% ethanol and then flaming the tops. 
2. If needed, exchange the gas in each test tube for 80% N₂ 20% CO₂ (do not over-pressurize; keep the pressure below 5 PSI). 
3. Thaw the frozen vial at room temperature under a gentle stream of oxygen-free gas. 
4. For inoculation, use a 1.0 ml syringe tipped with 22-gauge needle; withdraw the cell suspension from the vial and transfer it to the broth tube.  Transfer 0.5 ml of the culture to an additional tube of #2697 broth.   Plate 0.1 ml of the inoculated culture onto a non-selective medium and incubate aerobically at 37^oC.  Use 0.1 ml of the inoculated culture to inoculate a nonselective aerobic broth.  Incubate the broth tubes at 55^oC. 
5. Growth should be detected in the #2697 broth within 2 to 3 days.  There should be no growth detected on the aerobic plate or in the aerobic broth.

Additional information on this culture is available on the ATCC^© web site at www.atcc.org.

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