Streptococcus agalactiae Lehmann and Neumann

1. Open vial according to enclosed instructions. 
2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate #44 or #260 agar slants and/or plates.
5. Incubate the tubes and plate at 35-37°C for 24-48 hours.

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