Terrisporobacter mayombei (Kane et al.) Gerritsen et al.

1. Open vial according to enclosed instructions.
2. Under anaerobic conditions, withdraw 0.5 ml of #1053 from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth tube.  Additional tubes may be inoculated with 0.5 ml each from the suspension.  A slant of #1053 may also be inoculated with 0.2 ml.  Streak several blood plates to check for colonial morphology and purity.
4. Incubate tubes under an anaerobic atmosphere at 37°C.  Incubate one agar plate anaerobically for colony formation, and one aerobically for aerobic contamination check. 

ANAEROBIC CONDITIONS:   

Anaerobic conditions for transfer may be obtained by either of the following:

• Use of an anaerobic gas chamber, or  
• Placement of test tubes under a gassing cannula system hooked to anaerobic gas.   

Anaerobic conditions for incubation may be obtained by any of the following:

• Loose screw caps on test tubes in anaerobic chamber,                       
• Loose screw caps on test tubes in an activated anaerobic gas pack jar, or   
• Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.  

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