Rhodoplanes cryptolactis

1. Put 6 to 8 mL of Medium #112 into a small screw cap test tube (13x100 mm). Add 0.1 mL of 3% cysteine per each 56 mL of medium and then fill the test tube to capacity with additional Medium#112. Seal the test tube with a screw cap.
2. Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.
3. Open the freeze dried vial according to enclosed instructions.
4. Aseptically take 0.5 mL of the prereduced medium and rehydrate the pellet.
5. Transfer the rehydrated pellet back into the screw cap test tube and close tightly. (The test tube should be filled to capacity). Streak one drop of the rehydrated pellet onto Nutrient Agar (BD 213000)and/or Tryptic Soy Agar (BD 236950).
6. Incubate the broth culture at 30°C under a tungsten lamp. Incubate the aerobic Nutrient Agar plateand/or Tryptic Soy Agar plate at 37°C in the dark.
7. Once growth has been established (three to six days), the culture should be transferred to fresh broth. If a large inoculum is used (1020%)the medium does not need to be prereduced. Growth should be detected on the Nutrient Agar plate and/or Tryptic Soy Agar plate within 3 to 6 days.
8. When examined microscopically, the cells are motile rods, in single and pairs. When grown in broth, the culture is pink in color.

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