Pseudoalteromonas denitrificans (Enger et al.) Gauthier et al.

1. All media must be cooled to 10°C prior to opening vial. Keep media on ice while inoculating. Open vial according to enclosed instructions
2. Thaw and aseptically transfer the entire contents of the vial to a tube of fresh #1526 broth (5 to 6 mL).  Mix well.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Quickly incubate the tubes and plate at 10°C for 48 hours.  Utilizing a shaker will produce dense growth.

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