Campylobacter sputorum biovar sputorum On et al.

1. Open vial according to enclosed instructions.
2. Using a single tube of #1115 or #177 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette.  Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube.  Mix well.
4. Use several drops of the suspension to inoculate a #260 slant, and/or plate.
5. Or, to obtain a biphasic culture, add 0.5 mL of the suspension to a #260 agar slant (see notes).
6. Incubate tubes and plate at 37°C under microaerophilic conditions for three to five days.  Use an anaerobe jar with an active catalyst and a microaerophilic gas generator pack, or other acceptable method.  Incubate slant with cap loose. 

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