Sulfurimonas denitrificans
33889 ™
Download Genome Learn about the ATCC Genome Portal- Product category
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Bacteria
- Product type
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Extremophile
- Strain designation
- DSM 1251
- Type strain
- Yes
- Genome sequenced strain
- Yes
- Isolation source
- Estuarine mud
- Geographical isolation
- Netherlands; Dollard
- Applications
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Bioinformatics
- Product format
- Freeze-dried
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
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Biotechnology
- Preceptrol
- No
Characteristics
- Extremophile type
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Sulfur oxidizer
- Comments
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Genome sequenced strain
Handling information
- Medium
- Temperature
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26°C
- Handling procedure
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1. Open vial according to enclosed instructions.
2. Withdraw 0.5 ml of the recommended broth from a single test tube (5 to 6 ml) and rehydrate the entire vial contents.
3. Aseptically transfer this aliquot back into the broth. Additional broth tubes may be inoculated with 0.5 ml each from the suspension. A slant and a plate of the recommended medium may be inoculated with 0.2 ml each. A plate of non-selective medium such as nutrient agar may be streaked to check for aerobic contamination.
4. Incubate tubes and one plate under an anaerobic atmosphere at 26oC. Incubate non-selective plate aerobically to check purity.
5. In 3 to 6 days, growth should be evident by light turbidity in the broth and by colonies on the anaerobic agar surfaces. No growth should occur on the non-selective plate incubated in air. Colonies are small, circular, entire, convex, and translucent. Cells include slender rods of variable length and some long spirals.
ANAEROBIC CONDITIONS:
Anaerobic conditions for transfer may be obtained by either of the following:
· Use of an anaerobic gas chamber, or
· Placement of test tubes under a gassing cannula system hooked to anaerobic gas.
Anaerobic conditions for incubation may be obtained by any of the following:
· Loose screw caps on test tubes in anaerobic chamber,
· Loose screw caps on test tubes in an activated anaerobic gas pack jar, or
· Use of sterile butyl rubber stoppers on test tubes so that an anaerobic gas headspace is retained.
- Handling notes
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For best growth use freshly prepared media or media which has been stored under anaerobic conditions after preparation. Perform transfers under anaerobic conditions or minimize exposure to oxygen.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
Quality control specifications
- Verification method
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Whole-genome Sequencing
History
- Deposited as
- Thiomicrospira denitrificans Timmer-ten Hoor
- Depositors
- DSM
- Chain of custody
- ATCC <-- DSM <-- A. Timmer-ten Hoor
- Type of isolate
- Environmental
- Cross references
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GenBank CP000153 Sulfurimonas denitrificans DSM 1251, complete genome.
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
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The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
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This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.
type strain
Takai K, et al. Sulfurimonas paralvinellae sp. nov., a novel mesophilic, hydrogen- and sulfur-oxidizing chemolithoautotroph within the Epsilonproteobacteria isolated from a deep-sea hydrothermal vent polychaete nest, reclassification of Thiomicrospira denitrificans as Sulfurimonas denitrificans comb. nov. and emended description of the genus Sulfurimonas. Int. J. Syst. Evol. Microbiol. 56(Pt 8): 1725-1733, 2006. PubMed: 16901999
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