Cereibacter sphaeroides (van Niel) Hördt et al.

1. Put 6 to 8 mL of ATCC Medium #550 into a 13x100 mm screw cap test tube (small). Add cysteine (3.0% stock concentration, 2 mL/100 mL medium) and then fill the test tube to capacity with ATCC broth Medium #550. Seal the test tube with a screw cap.
2. Let the tube sit at room temperature for 30 minutes before inoculating it with the rehydrated culture.
3. Open the freeze-dried vial according to enclosed instructions.
4. Aseptically take 0.25 mL of the pre-reduced medium and rehydrate the pellet.
5. Transfer the rehydrated pellet back into the screw cap test tube and close tightly. One or two drops can be streaked out onto nutrient plates (ATCC Medium #3).
6. Incubate the broth culture at 30°C under a tungsten lamp. The nutrient agar plate cultures should be incubated aerobically in the dark. 
7. Growth should be established in three to six days. The culture should then be transferred to fresh broth. If a large inoculum is used the medium does not need to be pre-reduced.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.