Saccharomyces cerevisiae resources at ATCC
Transformation hosts and strains of various genotypes
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Search the catalogs based on any text word or word segment, including gene symbols and/or strain designations.
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Mapping membranes kit derived from clones from Saccharomyces cerevisiae AB972 (ATCC 76269)
This kit consists of the following elements:
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2 hybridization membranes containing DNA from physically mapped clones from the genome of Saccharomyces cerevisiae AB972. One membrane (labeled with L followed by a number) is derived from clones in bacteriophage lambda vectors. The other (labeled with C followed by a number) is from cosmid clones. For the most part, the clones are arrayed on both membranes in rough chromosome order.
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A tube containing 200 ng dried DNA to use as a positive control (labeled ATCC 57093).
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2 floppy disks, one formatted for IBM-PC compatible computers (labeled ATCC 77284: DOS disk) and one formatted for Macintosh computers (labeled ATCC 77284: MAC disk). These disks contain information you will need to use these membranes and interpret your results. You may use the editor, word-processing, or other software on your computer to read, search, and/or print this information. Please be aware that the length of a line of text for some of these files extends beyond 80 characters, so that you may not see all the information on your screen.
The same information is available in files at our ftp site.
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Genomic clones from S. cerevisiae AB972
Plasmid, cosmid, and bacteriophage lambda clones constructed in the laboratory of Dr. Maynard Olson (Proc. Natl. Acad. Sci. USA 83: 7826-7830, 1986, Genetics 106:681-698, 1991, Genetics 134:81-150, 1993).
Cosmids used to sequence yeast chromosomes (Science 265:2077-2082, 1994).
To find a clone for an ORF or gene, use the physical map search utility at the Saccharomyces Genome database and search on a gene symbol, ORF identifier, or clone.
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Enter your query on this form and select Physical Map as the display option. (The clones are represented by the bar labeled with a 5 digit number starting with 7.)
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Search clones category of our catalog and the Saccharomyces Genome Database (SGD) to obtain clone-based information.
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Other genomic and cDNA clones
Search the online catalog identify the clones you need.
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At that form, click on clones to select that category of our online catalog data.
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Enter other qualifying terms in the box labeled enter text to search.
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For example, to identify clones of Schizosaccharomyces pombe ura4, click on clones and enter query terms linked by the word and, for example: pombe and ura4.
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Vectors
pRS series vectors
Saccharomyces cerevisiae marker deletion plasmids
To find individual vectors, search the online catalog to identify the vectors you need.
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At that form, select vectors as the category of our catalog data.
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Enter other qualifying terms in the box labeled enter text to search.
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For example, to identify fragmentation vectors for S. cerevisiae, click on vectors and enter query terms linked by the word and, for example: cerevisiae and fragmentation.
Diagrams for some vectors are available.
FTP access to sequences for some vectors is available (files named by vector name).
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Vector sets or kits
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ATCC distributes sets of vectors for S. cerevisiae that differ by application and the selectable marker.
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Genomic and cDNA libraries
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At that form, select library and enter other qualifying terms in the box labeled enter text to search.
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For example, to identify yeast libraries deposited by S. Elledge, click on libraries and enter query terms linked by the word and, for example: yeast and Elledge.
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Other useful sites for information about Saccharomyces cerevisiae
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