Advanced Search

Quantitative Synthetic Human immunodeficiency virus 1 (HIV-1) RNA (ATCC® VR-3245SD™)

Product Format: frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable

Strain Quantitative Synthetic Human immunodeficiency virus 1 (HIV-1) RNA
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load and for other molecular biology applications.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 µL per vial with Biomatrica RNAstable
Storage Conditions -70°C or colder
Intended Use The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

Biomatrica Logo
Comments Manufactured under ISO 13485 guidance

Preparation includes fragments from the 5' LTR, gag gene, pol gene (including protease, reverse transcriptase, and integrase regions), tat gene, rev gene, and nef gene.

This construct also includes clinically significant mutation profiles to allow compatibility with the CDC genotyping assay patent, including 41L, 67N, 70R, 103N, 181C, 215Y, and 90M. (Centers for Disease Control and Prevention. Real-time PCR point mutation assays for detecting HIV-1 resistance to antiviral drugs. US Patent 8,592,146 dated Nov. 26, 2013)
Name of Depositor ATCC
U.S. Patent Number 8,592,146
Special Collection RNA
References

Henrich TJ, et al. Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCR. J. Virol. Methods 186(1-2): 68-72, 2012. PubMed: 22974526

Centers for Disease Control and Prevention. Real-time PCR point mutation assays for detecting HIV-1 resistance to antiviral drugs. US Patent 8,592,146 dated Nov. 26, 2013

Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
SDS
SDS
FAQs
  1. Test primers and probe for HIV synthetic RNA VR-3245SD

    ATCC used the following primers and probe to obtain a positive PCR product from this RNA.


    Date Updated: 8/25/2015