Product Description

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ATCC® Primary Cell Solutions™ Media, Supplements and Reagents
ATCC® Number: PCS-300-040    Price: £79.00; €85.00
Description: Bronchial Epithelial Cell Growth Kit
Quantity: 1 kit
Biosafety Level: 1
Shipped: frozen
Storage: -20°C (or -70%deg;C for long-term storage)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Bronchial Epithelial Cell Growth Kit contains components that when added to Airway Epithelial Cell Basal Medium (ATCCPCS-300-030) create a complete ATCC® Primary Cell Solutions™ culture environment for bronchial/tracheal epithelial cells derived from normal human lung. The serum-free medium formulation is designed to support normal airway epithelial cell morphology as well as promote rapid growth and proliferation. No feeder layers, extracellular matrix proteins or other substrates are required.

The final concentration for each component in complete bronchial/tracheal epithelial cell growth medium is as follows:
HLL Supplement: HSA 500 µg/ml, Linoleic Acid 0.6 µM, Lecithin 0.6 µg/ml
L-glutamine: 6 mM
Extract P: 0.4%
Airway Epithelial Cell Supplement: Epinephrine 1.0 µM, Transferrin 5 µg/ml, T3 10 nM, Hydrocortisone 5 µg/ml, rh EGF 5 ng/ml, rh Insulin 5 µg/ml
Related Products: Primary Bronchial/Tracheal Epithelial Cells; Normal, Human (ATCCPCS-300-010)

Airway Epithelial Cell Basal Medium (ATCCPCS-300-030)

Dulbecco's Phosphate Buffered Saline, (D-PBS) (ATCC30-2200)
Trypsin-EDTA for Primary Cells (ATCCPCS-999-003)
Trypsin Neutralizing Solution (ATCCPCS-999-004)
Gentamicin-Amphotericin B Solution (ATCCPCS-999-025)
Penicillin-Streptomycin-Amphotericin B Solution (ATCCPCS-999-002)
Phenol Red (ATCCPCS-999-001)
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