Product Description

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Cell Biology
ATCC® Number: CRL-3002™    Price: £588.00; €698.00
Designations: JVM-2
Depositors:  J.V.Melo
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast

Characterization Data
Source: Organ: peripheral blood
Cell type: lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed
Disease: Mantle Cell Lymphoma
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Oncogene: p53 [PubMed: 16434365]
Antigen Expression: CD3-, CD5-, CD10-, CD19+, CD20+, CD23+ (verified at ATCC)
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17
Cytogenetic Analysis: Bimodal chromosome number: 46 and 92 This is a bimodal female cell line with approximately half the cells being pseudodiploid and the other half pseudotetraploid. Most cells contain the t(11;14)(q13;q32) derivative chromosome common to Mantle Cell Lymphoma. Other derivative chromosomes included: t(3;8)(q13;p21), add(4q) [or t(4;13)(q2?5;q1?4)], del(13)(q21) and dup(16p).
Age: 63
Gender: female
Ethnicity: Caucasian
Comments: Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA. JVM-2 is characterized cytogenetically by t(11;14)(q13; q32) [PubMed: 3093393 and 3262465]. JVM-2 cells retain p16 expression and exhibit low expression of cyclin D1. This cell line expresses cyclin D2 [PubMed: 16183118]. Based on the new classification of lymphomas, JVM-2 is considered a Mantle Cell Lymphoma (MCL) [PubMed: 16183118 and PubMed: 15229187].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(4) to 3 x 10(4) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Preservation: 70% growth medium; 20% FBS; 10% DMSO
Doubling Time: about 48 hours
References: 92892: Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393
92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172638: de Leeuw RJ, et al. Comprehensive whole genome array CGH profiling of mantle cell lymphoma model genomes. Hum Mol Genet.: 1;13(17):1827-37 ( 2004). PubMed: 15229187.
16172639: Tucker, C.A., et al. Four human t(11;14)(q13;q32)-containing cell lines having classic and variant features of Mantle Cell Lymphoma. Leuk Res. 30(4): 449-457, 2006. PubMed: 16183118.
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