Product Description

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Vector
ATCC® Number: 87400™ Price: £228.00; €298.00
Designations: pBAD28
Depositors: L Guzman, J Beckwith
Biosafety Level: 1
Vector Information: Size (kb): 5.8010001182556150
Vector: pBAD28 (phagemid)
Promoters: Promoter araC
Construction: pBAD18, pACYC184
Marker(s):ampR,cmlR,araC
Construct size (kb): 5.801000118255615
Features: marker(s): ampR
marker(s): cmlR
operator: I2 + I1
operator: O1
operator: O2
other: CAP site
promoter: araC
promoter: bla (ampR)
promoter for expression: arabinose BAD
regulator: araC
replicon: M13
replicon: p15A
MCS: NheI...HindIII
transcription terminator: rrnB T1 + T2
Map
Shipped: freeze-dried plasmid in E. coli
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: expression vector [45845]
produces protein arabinose regulator [45845]
vector containing primer sites useful for sequencing [45845]
vector with low copy number [45845]
Comments: Restriction digests of the clone give the following sizes (kb): AvaI--3.1, 1.9, 1.3; BamHI--6.1; PstI--5.0;1.2.
Cultures should be grown in minimal media for more reproducible induction of expression. Expression is induced in glycerol-containing media by addition of arabinose. Expression is repressed by addition of glucose or other catabolites. [45845]
One of several tightly controlled expression vectors (ATCC87393-87402) regulated by the arabinose operon. The vectors differ in replicon, antibiotic resistance marker, multiple cloning site and mechanism of initiation of translation. [45845]
The following primers can be used for sequencing of cloned inserts: 5' primer (27 - 8 bp upstream of the NheI site) 5'-CTGTTTCTCCATACCCGTT-3'; and one of two 3' primers: 3' primer 1 (2 - 19 bp downstream of the HindIII site) 5'-CTCATCCGCCAAAACAG-3'; [45845]
3' primer 2 (17 - 33 bp downstream of the HindIII site) 5'-GGCTGAAAATCTTCTCT-3'. [45845]
Plasmid copy number is low due to the p15A replicon. This vector can be used when reduced gene expression is desirable. [45845]
Plasmid is compatible with pBR-derived plasmids and may be used for coexpression of cloned inserts. [45845]
Growth Conditions: ATCC medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin
Temperature: 37.0°C
References: 45845: Guzman LM, et al. Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. J. Bacteriol. 177: 4121-4130, 1995. PubMed: 7608087
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