Traditional attention to quality, research and science makes ATCC your most reliable source for stem cell lines and reagents. You can browse the stem cell lines, media and reagents below and click on the link to view the full product description and then request materials online. Click here to download a portfolio of available ES cells. These materials are also incorporated into ATCC's online catalog.
Mouse ES Cells
Our collection includes many of the most influential and widely cited mouse ES cell lines (D3, AB lines, J1, R1, R1/E, E14 derivatives). The cells are tested for markers associated with pluripotency (Oct 3/4) and to assure that the cells remained undifferentiated (alkaline phosphatase activity and SSEA-1 expression) during expansion.
| ATCC® Number |
Product Name |
Description |
| CRL-1934 |
ES-D3(D3) |
Derived from strain 129S2/SvPas mouse blastocyst |
| SCRC-1002 |
ES-C57BL/6 |
Derived from strain C57BL/6J (B6) mouse blastocyst |
| SCRC-1010 |
J1 |
Derived from a male agouti 129S4/Svjae embryo; useful in studies on embryonic development and Cre-Lox recombination |
| SCRC-1011 |
R1 |
Established from a 3.5 day blastocysts produced by crossing two 129 mouse substrains (129S1/SvlmJ and 129X1/Svj) |
| SCRC-1016 |
ESF 158 |
Developed from (NOD×129) F1 × 129 backcross 1 mice, intercrossed to select for homozygous regions containing disease loci; useful for genetic targeting of the nonobese diabetic (NOD) mouse genome |
| SCRC-1018 |
RW.4 |
Derived from a strain 129X1/SvJ mouse blastocyst; useful for gene knock-out/knock-in and studies related to differentiation and ESC proliferation |
| SCRC-1019 |
B6/BLU |
Derived from a C57BL/6 transgenic line containing a LacZ reporter; the transgene is a beta-globin LacZ fusion |
| SCRC-1020 |
SCC#10 |
Derived from a strain 129Sv/J (129X1Sv/J) mouse blastocyst; useful for gene knock-out/knock-in |
| SCRC-1021 |
EDJ#22 |
Derived from 129SvEV mice from Taconic; useful in gene targeting experiments |
| SCRC-1023 |
AB2.2 |
Originated from a Steel substrain of 129 mice (129/SvEvBrd-Hprtb-m2); useful in recombineering with a unique, fully end-sequenced, 129Sv BAC library generated from AB2.2 ES cell DNA |
| SCRC-1033 |
7AC5/EYFP |
A yellow fluorescent variant of R1 ES cells (129S1/SvImJ and 129X1/SvJ) generated by the random integration of EYFP using co-electroporation with a circular selectable marker containing vector pPGK Puro |
| SCRC-1036 |
R1/E |
Subcloned from R1 ES cells (129S1/SvImJ and 129X1/SvJ) |
| SCRC-1037 |
G-Olig2 |
Strain 129X1/SvJ ES line designed by the insertion of green fluorescent protein (GFP) into the gene for Olig2; the insertion permits visualization and physical separation of a subset of living ES-cell-derived neural cells |
| SCRC-1038 |
CE-1 |
Derived from the D3 ES line; CE1 (for Cassette Exchange) contains one ‘acceptor’ module that allows for efficient double lox targeting (the cells are hygromycin resistant) |
| SCRC-1039 |
CE3 |
Derived from D3 ES line; CE3 (for Cassette Exchange) contains one ‘acceptor’ module that allows for efficient double lox targeting with constitutive GFP expression (the cells are puromycin resistant) |
Human ES Cells
| ATCC® Number |
Product Name |
Description |
| SCRC-2002 |
hESC BG01V |
BG01V cells are pluripotent and can differentiate to representatives of the three primay germ layers |
Support Cells and Reagents for Feeder-Dependent Culture
Our classic MEF feeder cell lines, including our new antibiotic-resistant lines, are tested and validated on multiple stem cell lines for safe and robust stem cell culture. In response to customer requests, we now offer Mitomycin C, an antineoplastic antibiotic, along with the ATCC recommended protocol to prepare inactivated feeder cells.
| ATCC® Number |
Product Name |
Description |
| SCRR-3020 |
Mitomycin C |
Antineoplastic antibiotic to prepare inactivated feeder cells |
| 56-X |
IRR-STO |
Irradiated STO cells |
| CRL-2581 |
C166 |
Mouse embryonic endothelial cell |
| CRL-2582 |
C166-GFP |
Mouse embryonic endothelial cell with GFP expression |
| CRL-2749 |
OP9 |
Mouse embryonic bone marrow stromal cells |
| SCRC-1007 |
AFT024 |
Mouse embryonic liver fibroblast |
| SCRC-1007.1 |
AFT024 IRR |
Irradiated mouse embryonic liver fibroblast |
| SCRC-1008 |
MEF (C57BL/6)[MEF-BL/6-1] |
Mouse embryonic fibroblast |
| SCRC-1008.1 |
MEF (C57BL/6) IRR |
Irradiated mouse embryonic fibroblast |
| SCRC-1008.2 |
MEF (C57BL/6) MITC |
Mitomycin C treated mouse embryonic fibroblast |
| SCRC-1040.2a |
MEF (CF-1) MITC |
Mitomycin C treated mouse embryonic fibroblast |
| SCRC-1040 |
MEF (CF-1) |
Mouse embryonic fibroblast |
| SCRC-1040.1 |
MEF (CF-1)IRR |
Irradiated mouse embryonic fibroblast |
| SCRC-1045 |
MEF (DR4) |
Multi-drug resistant mouse fibroblast |
| SCRC-1041 |
HFF-1 |
Human foreskin fibroblast |
| SCRC-1041.1 |
HFF-1 IRR |
Irradiated human foreskin fibroblast |
| SCRC-1041.2 |
HFF-1 MITC |
Mitomycin C treated human foreskin fibroblast |
| SCRC-1049 |
SNL76/7 |
Mouse STO fibroblast with G418 resistance and endogenous expression of LIF |
| SCRC-1050 |
SNL76/7-4 |
Mouse STO fibroblast with resistance to G418 and puromycin plus endogenous expression of LIF |
Classic Media, Supplements and Reagents to Support
Feeder-Dependent Culture
Standardized for reproducible results, our ES-cell qualified media and FBS support the most frequently used culture conditions.
| ATCC® Number |
Description |
| SCRR-2010 |
ES-DMEM (Dulbecco's Modified Eagle's Medium, optimized for murine ES cells) |
| SCRR-2011 |
Mouse ES Cell Basal Medium |
| SCRR-2201 |
Phosphate Buffered Saline Without Calcium or Magnesium |
| SCRR-30-2020 |
Fetal Bovine Serum, ES Cell Qualified |
| 30-2006 |
DMEM:F12 Medium |
| 30-2115 |
L-Alanyl-L-Glutamine, 200 mM Solution in 0.85% NaCl |
| 30-2116 |
MEM Nonessential Amino Acid Solution, 100x |
| 30-2101 |
Trpsin EDTA Solution |
If you have questions about the ATCC stem cell products, contact us. |