STR Database for Human Cell Lines
As part of our continuing efforts to characterize and authenticate the cell lines in the Cell Biology collection, ATCC is developing a comprehensive database of short tandem repeat (STR) DNA profiles for all of our human cell lines.
STR loci are among the most informative polymorphic markers in the genome. ATCC generates the STR profile by simultaneously amplifying eight STR loci, plus the amelogenin gene for gender determination, in a multiplex PCR reaction (Promega PowerPlex® 1.2 system), enabling a 1 in 108 discrimination rate for unrelated individuals.1 The amplicons are separated by capillary electrophoresis and analyzed using Genemapper® ID 3.2.1 software from Applied Biosystems. Each peak in the resulting electropherogram represents an allele which is alphanumerically scored and entered into the database.
As in the past, when we find a misidentified cell line among our holdings (i.e., the DNA profile is similar or identical to that of an unrelated cell line), we will post a note on the Misidentified Cell Lines page on our website. We hope this database will facilitate discussions on cell line authenticity.
STR profiles are provided to the scientific community to help ensure the quality and integrity of your cell lines. After determining the STR profiles of your human cell lines, you may compare them to the ATCC human cell line collection. If you have any questions about STR loci or this database, please contact a technical service representative.
Please define your search by selecting either an ATCC number OR by entering the numeric values of each allele designation below.
Note: You will need to enter at least 7 of the 8 STR loci in order to return any possible matches. Amelogenin is not a STR but a marker used for gender determination.
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